Real-time detection of PRT1-mediated ubiquitination via fluo-
نویسندگان
چکیده
1 Independent Junior Research Group on Protein Recognition and Degradation, Leibniz Institute of Plant Biochemistry 8 (IPB), Weinberg 3, D-06120 Halle (Saale), Germany 9 2 ScienceCampus Halle – Plant-based Bioeconomy, Betty-Heimann-Str. 3, D-06120 Halle (Saale), Germany 10 3 Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry (IPB), Weinberg 3, D-06120 Halle (Saale), 11 Germany 12 4 present address: Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 Arany Janos, Cluj-Napoca, 13 Romania 14 5 present address: Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany 15 16 17 * Correspondence should be addressed to N.D. ([email protected]). 18 19 20 21 RUNNING TITLE 22 Live assays with fluorescent N-end rule substrates 23
منابع مشابه
Real‐time detection of N‐end rule‐mediated ubiquitination via fluorescently labeled substrate probes†
The N-end rule pathway has emerged as a major system for regulating protein functions by controlling their turnover in medical, animal and plant sciences as well as agriculture. Although novel functions and enzymes of the pathway have been discovered, the ubiquitination mechanism and substrate specificity of N-end rule pathway E3 ubiquitin ligases have remained elusive. Taking the first discove...
متن کاملCopper concentration using micelle-mediated extraction in real samples
A simple and sensitive method is described for the pre-concentration by cloud point extraction followed by atomic absorption spectrometric determination of copper ion in real samples. At a solution pH of 5.5, 5, 5-diphenylimidazolidine-2, 4-dione (phenytoin) (DFID) and TritonX-114 were used as hydrophobic ligand and non-ionic surfactant. The adopted concentrations for DFID, Triton X-114 and HNO...
متن کاملDAMAGE DETECTION OF BRIDGE STRUCTURES IN TIME DOMAIN VIA ENHANCED COLLIDING BODIES OPTIMIZATION
In this paper, a method is presented for damage detection of bridges using the Enhanced Colliding Bodies Optimization (ECBO) utilizing time-domain responses. The finite element modeling of the structure is based on the equation of motion under the moving load, and the flexural stiffness of the structure is determined by the acceleration responses obtained via sensors placed in d...
متن کاملInvestigation of Efflux-Mediated Tetracycline Resistance in Shigella Isolates Using the Inhibitor and Real Time Polymerase Chain Reaction Method
Background: Shigella spp. are gram negative bacteria, which are of global public health importance. The growing of multidrug-resistant Shigella isolates are a major problemaround the world. Methods: Overall, 50 isolates of Shigella spp. from children diarrheic stools w...
متن کاملDifferential ubiquitination and degradation of huntingtin fragments modulated by ubiquitin-protein ligase E3A.
Ubiquitination of misfolded proteins, a common feature of many neurodegenerative diseases, is mediated by different lysine (K) residues in ubiquitin and alters the levels of toxic proteins. In Huntington disease, polyglutamine expansion causes N-terminal huntingtin (Htt) to misfold, inducing neurodegeneration. Here we report that shorter N-terminal Htt fragments are more stable than longer frag...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016